Methods in Common Use for Detecting/quantifying Transcripts 1. Northern blotting and in situ hybridization * RNAse protection assays * cDNA microarrays 4. Reverse transcription-polymerase chain reaction with end-point or real-time quantitation. Quantitation by RT-PCR RNA extraction RNA extraction Reverse Transcription Reverse Transcription cDNA to PCR rx PCR reaction Electrophoresis/ Dot Blots/imaging Image analysis cDNA to [...]
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